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Effect of virulence and serial transfers of Xanthomonas campestris on xanthan gum production BJM
Nitschke,Marcia; Rodrigues,Vanessa.
The virulence of six Xanthomonas campestris isolates was evaluated using the percentage of lesion area of leaves in Brassica oleraceae host plant, compared to diameter of colonies, xanthan production and gum viscosity. In terms of virulence, the isolates belonged to two statistically different groups: isolates B, UPF and C7 showed values between 52 and 69%, while isolates CF, C and strain B-1459 gave 0-30% of lesion area. Final xanthan concentration, gum viscosity and colony diameter did not correlate with virulence calculated by percentage of lesion area, showing that this parameter is not a good criterium for selection of potential xanthan producer isolates. Serial transfers of X. campestris isolates in host plant did not show a significant effect on "in...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xanthomonas campestris; Serial transfers; Virulence; Xanthan.
Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000100014
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Entrapment of anaerobic thermophilic and hyperthermophilic marine microorganisms in a gellan/xanthan matrix ArchiMer
Landreau, M.; Duthoit, Frederique; Claeys-bruno, M.; Vandenabeele-trambouze, O.; Aubry, T.; Godfroy, Anne; Le Blay, Gwenaelle.
Aims The aims of this study were (i) to develop a protocol for the entrapment of anaerobic (hyper)thermophilic marine micro-organisms; (ii) to test the use of the chosen polymers in a range of physical and chemical conditions and (iii) to validate the method with batch cultures. Methods and Results The best conditions for immobilization were obtained at 80°C with gellan and xanthan gums. After 5-week incubation, beads showed a good resistance to all tested conditions except those simultaneously including high temperature (100°C), low NaCl (<0∙5 mol l−1) and extreme pH (4/8). To confirm the method efficiency, batch cultures with immobilized Thermosipho sp. strain AT1272 and Thermococcus kodakarensis strain KOD1 showed an absence of detrimental effect...
Tipo: Text Palavras-chave: (hyper)thermophilic marine micro-organisms; Anaerobiosis; Entrapment; Gellan; Immobilization; Xanthan.
Ano: 2016 URL: http://archimer.ifremer.fr/doc/00332/44352/44000.pdf
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Immobilisation et culture continue en bioréacteur gas-lift de microorganismes marins thermophiles et hyperthermophiles anaérobies ArchiMer
Landreau, Matthieu.
Since the discovery of hydrothermal vents, multiple studies have been conducted in order to study microbial diversity. Molecular inventories realized have thus demonstrated a great diversity of species that contrasts with the low proportion (1%) of species isolated by culture approach. A new cell immobilization approach by inclusion in a polymer matrix (gellan and xanthan) has been developed for the study of these thermophilic anaerobic marine communities. The system, based on the formation of an emulsion between an inoculated polymer solution and oil, allows the entrapment of cells in gel beads with a diameter between 1 and 2 mm. The optimal immobilization conditions were obtained for emulsion performed at 80 °C with stirring (150 rpm) with a polymer...
Tipo: Text Palavras-chave: Immobilisation cellulaire; Gellane; Xanthane; Site Rainbow; Culture continue; Cell immobilization; Gellan; Xanthan; Rainbow site; (hyper)thermophile; Continuous culture.
Ano: 2016 URL: https://archimer.ifremer.fr/doc/00498/60933/64327.pdf
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Technological application of an extracellular cell lytic enzyme in xanthan gum clarification BJM
Shastry,Suresh; Prasad,M.S..
An extracellular cell lytic enzyme from Pseudomonas sp. was active on heat killed cells of Xanthomonas campestris. The lytic activity caused enzymatic digestion of X.campestris xanthan gum. Digestion was effective for highly viscous native xanthan 2.0% (w/v) and 2.5% (w/v) commercial Sigma xanthan. Scanning electron microscopy and SDS-PAGE observations confirmed the cell lytic action on X.campestris cells.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Xanthan; Cell lytic enzyme.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000100012
Registros recuperados: 4
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